What Type of Service?
We want to make your project quick and seamless.
Sequence Design Service
It is important to start with the right protein sequence. We can help design the best sequence for your experiment by reading relevant articles, adding fusion proteins or tags, and ordering the correctly cloned DNA sequence. We can send the DNA plasmid to you or keep it here at Solidzymes for your protein expression project.
expression and purification Service
Solidzymes offers custom protein expression and purification from E. Coli. We measure protein expression levels under different conditions such as media type, temperature, and inducer concentration Similarly, we can vary the chromatography conditions used during purification. The final result is a protein expression and purification protocol that you know works for your protein.
High Density expression and Purification
We offer protein expression in a five liter benchtop fermenter. This allows E. Coli cultures up to the equivalent of fifty liters of shake flask culture. High-density fermentation is a great way to produce grams of protein for large experiments or for many small experiments that need a consistent supply of protein from your freezer. We also offer freeze drying for improved shipping and storage of your protein.
| Service | Deliverables | Time | Estimate |
|---|---|---|---|
| Protein construct design and synthesis | Codon optimized DNA sequence and sample | 2 – 3 weeks | $400 |
| Protein test expression in E. Coli | Image of SDS-PAGE and frozen cells | 2 days | $200 |
| Protein production – shaker | Image of SDS-PAGE and frozen cells | 1 week | $500 |
| Protein production – fermenter | Image from SDS-PAGE and frozen cells | 1 week | $5000 |
| Protein purification – His tag | Image from SDS-PAGE and frozen protein | 1 day | $500 |
| Protein purification – ion exchange | Image from SDS-PAGE and frozen protein | 1 day | $700 |
| Protein purification – heat | Image from SDS-PAGE and frozen protein | 1 day | $400 |
| Soluble activity assay development | Description of optimized experimental setup | 1 week | Call for quote |
| Soluble activity condition optimization | Report on conditions for optimal enzyme activity | 1 – 2 weeks | Call for quote |
Enzyme activity Optimization
The conditions encountered by an enzyme during production, purification, and immobilization can have profound effects on the activity and useful lifetime of the resulting biocatalyst. We can test a variety of conditions at every stage of biocatalyst production – expression, purification, immobilization, and finally reaction. This way the protocol we ultimately develop for you is as cost-effective as possible.
Enzyme Carrier Screening
If you need to immobilize your enzymes then it is crucial to select the right enzyme immobilization support material. There are now hundreds of enzyme carriers available commercially, and many more on the horizon. At Solidzymes we are familiar with the industry and have a large selection of enzyme carriers from top brand names. Importantly, we have the unbiased expertise needed to match our customers to the best materials for their needs.
Immobilized Enzyme yield optimization
Testing immobilized enzyme activity can be challenging, but we have the experience to do it efficiently. Once a good enzyme carrier is identified we can test a variety of conditions for immobilization such as enzyme loading, pH, and temperature. We will re-use your new biocatalyst in batch mode or continuously operate it in a packed bed reactor format. We carefully compare the reaction rate and stability over time to identify the recipe that gets the most out of your enzymes.
| Service | Deliverables | Time | Estimate |
|---|---|---|---|
| Immobilized activity screening – 48 carriers | Report on binding and initial activity on each carrier | 2 weeks | $4,000 – $6,000 |
| Immobilized activity screening – >48 carriers | Report on binding and initial activity on each carrier | 2 – 3 weeks | $6,000 – $12,000 |
| Immobilization condition optimization | Report on conditions for optimal enzyme immobilization | 1 week | $20 / well |
| Immobilized stability followup | Report on immobilized enzyme stability | As needed | $500 / test |
| Immobilized activity assay development | Description of optimized experimental setup | 1 week | $3,000 – $4,000 |
| Immobilized enzyme reactor prototype | Report on yield from continuous production | ?? weeks | $10,000 – $20,000 |