-
Lactase Stabilization with Glutaraldehyde
Glutaraldehyde is a well known covalent crosslinker that is often used to improve the enzyme immobilization process by creating covalent attachment points (Rodrigues et al. 2021). It reacts with amine groups on the surface of proteins / particles and in so doing promotes structural integrity of the enzyme by connecting it to the solid support,…
|
-
Lactase Case Study
The beta-galactosidase enzyme, also known as lactase, is an industrial biocatalyst used to produce lactose free milk (Horner, 2011). The activity of this enzyme is easy to measure and it is commercially available (Biosynth FL31718) making it an excellent model to test Solidzymes’ carrier screening methodology and equipment. Enzyme carriers were first assessed for their…
|
-
Moisture Content Analysis.
Thanks to our suppliers we are up to forty five carriers in the Solidzymes library. These are commercially produced materials from brands like Doulite, Amberlite, Seplife, Indion, DIAION, Relizyme, and others. Most were provided wet, so in order to weigh out comparable amounts we need to know how much liquid is in the samples. We…
|
-
Carrier Equilibration
It is essential to properly equilibrate carriers with a buffer solution before presenting enzymes to the surface. The buffer should maintain a pH that promotes enzyme solubility throughout the immobilization interaction and in the following days, otherwise the final biocatalyst may not perform well. Since the ionic capacity of many enzyme carriers, especially anion exchange…
|
The Blog
Here you can read about current events and examples from finished projects.