-
Co-Immobilization of Enzymes: A Real Game Changer.
The first enzymatic processes developed at an industrial scale used one enzyme to accomplish one reaction. Over the years, more complex reactions have been conceptualized, involving multiple enzymes working sequentially or in concert to achieve a multi-step reaction. The need for multiple enzyme reactors is a burden many companies do not relish, and so enzyme…
|
-
Maximizing cost efficiency through enzyme immobilization
The need for affordable and sustainable approaches within industrial biotechnology continues to grow. One of the most revolutionary breakthroughs has been industrial enzyme immobilization. By adsorbing enzymes onto solid supports this technique boosts enzyme stability, allows enzyme reuse in the industry, and facilitates re-use in multiple reaction cycles. In addition, reducing the cost of goods…
|
-
Glucose-6-phosphate Dehydrogenase characterization
Expression of His tagged proteins in E. Coli followed by purification using nickel agarose beads is the method of choice to produce most proteins at Solidzymes. This is probably the most common method to produce recombinant proteins at lab scale, however, each lab does it a little differently. In this post we describe purification of…
|
-
Lactase Stabilization with Glutaraldehyde
Glutaraldehyde is a well known covalent crosslinker that is often used to improve the enzyme immobilization process by creating covalent attachment points (Rodrigues et al. 2021). It reacts with amine groups on the surface of proteins / particles and in so doing promotes structural integrity of the enzyme by connecting it to the solid support,…
|
-
Lactase Case Study
The beta-galactosidase enzyme, also known as lactase, is an industrial biocatalyst used to produce lactose free milk (Horner, 2011). The activity of this enzyme is easy to measure and it is commercially available (Biosynth FL31718) making it an excellent model to test Solidzymes’ carrier screening methodology and equipment. Enzyme carriers were first assessed for their…
|
Insights & Innovations
Here you can read about current events and examples from finished projects.